K-Series Centrifuges I. Development of the K-II continuous-sample-flow-with-banding centrifuge system for vaccine purification

Summary The K-II continuous-sample-flow-with-banding-9 zonal centrifuge hasbeen developed for large-scale virus isolation. The cylindrical aluminum rotor (capacity 3600 ml) contains a 3 liter gradient and has a 700 ml stream volume. Fluid line seals are located on opposite ends of the rotor, eliminating the possibility of cross-leakage. An air turbine drive is used to accelerate the rotor to 35,000 rpm (83,440 g , maximum). The development of safe armor and control systems is described.