Analysis of iodine-labelled glucagon derivatives by high-performance liquid chromatography

Abstract A reversed-phase high-performance liquid chromatographic system is presented for the separation of iodinated glucagon from the corresponding unlabelled and oxidized hormone, obtained by iodination of glucagon using the hydrogen perioxide—lactoperoxidase or the chloramine-T method. As the iodinated derivatives are well separated from the iodinated and oxidized as well as from the unlabelled glucagon molecules, this separation technique has been used both for optimizing the iodination procedures and for the rapid, efficient purification of mono- and diiodinated glucagon derivatives.