The effect of CXCL12/CXCR4 biological axis on biological behavior of pancreatic cancer cells

Objective The aim of this study is to explore the effect of CXCL12/CXCR4 biological axis on biological behavior of pancreatic cancer cells through the detection of mRNA expressions.Methods Pancreatic cancer cells (Miapaca-2) were cultured in vitro and divided into the control group,CXCL12 group and AMD3100 group.(1)RT-PCR was applied to detect the mRNA expressions of CXCL12,CXCR4,matrix metalloproteinase 2 (MMP-2),MMP-9 and human urokinase plasminogen activator (uPA) ; (2) CCK-8 methods was used to detect the proliferation of cells; (3) Transwell invasion assay was applied to detect the invasive ability of pancreatic cancer cells among groups respectively.Results The mRNA expression of CXCL12 was not found,while the mRNA expression of CXCR4 was observed in pancreatic cancer cells.There was a tendency of decreasing on the mRNA expressions of MMP-2,MMP-9 and uPA among three groups,and there was statistically significant difference (P ＜ 0.05).The proliferative and invasive ability of pancreatic cancer cells showed an enhanced trend in CXCL12 group,while in AMD3100 group was effectively inhibited.There was statistically significant difference among three groups (P ＜ 0.05).Conclusions CXCL12/CXCR4 biological axis plays an important role in proliferation and invasion of pancreatic cancer cells. Key words: Pancreatic neoplasms;  Biology;  Chemokines, CXCL12;  Receptors,CXCR4