Молекулярно-генетический анализ состава запасных белков в образцах коллекции пшенично-пырейных гибридов

The aim of the paper is to study the genes ofwheatgrass storage proteins in wheat-wheatgrass hybrids. The study was carried out using seeds of 26 lines of wheat-wheatgrass hybrids developed in the Department of Distance Hybridization of Tsitsin Main Botanical (Russian Academy of Sciences) using medium and ponticum wheatgrass. The analysis was performed using polymerase chain reaction (PCR), SDS-PAGE storage protein electrophoresis, PCR-product cloning, sequencing, and BLAST-analysis. 23 wheat-wheatgrass hybrids with the genes ofwheatgrass high-molecular (HMW) glutenins have been detected. The expression of these genes at least in 15 of 23 lines has been shown using protein electrophoresis. It can result from either the "silence" of the genes or the similarity or identity of the expressed wheat and wheatgrass protein subunits in molecular weight, so, they cannot be distinguished with one-dimensional electrophoresis. Wheatgrass storage protein genes of two wheat-wheatgrass lines (1670 and 197/2-1-2trs) were cloned and sequenced. As a result, two different nucleiс sequences have been described: 1152 b.p. for 1670 and 1352 b.p. for 197/2-1-2trs. BLAST-analysis of the sequences showed the relation of them to HMWglutenin genes of Th. intermedium and Th. elongatum. The gene revealed in 1670 is clasterized with the HMW glutenin gene ofTh. intermedium, and that of 197/2-1-2trs with Lophopyrum elongatum (syn. Th. elongatum). The found lines are interesting as an object to study the influence of wheatgrass HMW glutenins on gluten, storage proteins compound and bread-making quality.