A novel method to radiolabel stealth liposome through 1,2- dimyristoyl-sn-glycero-3-phosphoethanolamine-N-DTPA with 99mTc and biological evaluation

Purpose : To study surface technetium labeling of stealth DTPA-Liposome and to evaluate its potential as a molecular imaging tracer for both normal and melanoma-bearing mice. The radiolabeling yield of liposomes was greater than 90% and showed good chemical and biological stability. Biodistribution studies in normal mice showed blood clearance with hepatic and renal depuration. Melanoma-bearing mice showed a similar pattern of biodistribution with high tumor uptake allowing tumor imaging. The developed method of surface radiolabeled DTPA-PEG-Liposomes with 99m Tc was effective and stable in vivo .