Assessment of the Potential Role of Active Vitamin D Treatment in Telomere Length: A Case–Control Study in Hemodialysis Patients

Background: Telomeres are special chromatin sequenceslocatedattheendofeukaryoticchromosomes, protecting these regions from recombination and degradation. Previous studies have reported a decrease in telomerelengthonwhitebloodcellsfromhemodialysis (HD) patients, which suggests premature senescence. Active vitamin D treatment has been reported to have an effect on telomere length and beneficial effects on HD patients, but the mechanisms are unknown. Objective: Our aim was to assess the potential protective role of active vitamin D treatment on telomere length in peripheral mononuclear cells (PBMC) from HD patients. Methods: A retrospective case‐control study of 62 stable HD patients and 60 healthy sex-matched controls was undertaken. Telomere length was measured in PBMC by Southern blot. After telomere length measurement, 5 control samples that did not reach qualitycontrol standards were excluded. Standard epidemiological and biochemical parameters were recorded. Blood biochemistries were performed at the Biochemistry DepartmentoftheUniversityHospitalArnaudeVilanovain Lleida, Spain, using standard routine techniques. Differences in telomere length were analyzed using Student’s t test. Multiple regression analysis examined the independent contribution of the factors that significantly affected telomere length in the bivariate analysis. Results: HD patients presented shorter telomere length in PBMC, independent of age and sex (mean [SD] 8.8 [1.5] kbp vs 10.5 [2.9] kbp; P 0.0001). MultivariateregressionanalysisoftheHDsubgroupsuggestedthat patients under active vitamin D treatment have greater telomere length in PBMC than untreated patients (9.5 [0.2] kbp vs 8.4 [0.2] kbp; P 0.003). Conclusions: HD patients were observed to have decreased PBMC telomere length compared with healthy controls. HD patients treated with active vitamin D compounds had greater PBMC telomere length than untreated patients. Prospective studies are required to assess the potential role of active vitamin D treatment in PBMC telomere length. (Clin Ther. 2012; 34:849‐856) © 2012 Elsevier HS Journals, Inc. All rights reserved.