Primer, probe, kit and method for detecting A, B and H5 subtype influenza virus by triple fluorescent quantitative RT-PCR

The invention discloses a primer, a probe and a detection kit containing the primer and the probe, which are used for detecting A type, B type and H5 subtype influenza viruses through triple fluorescence quantitative RT-PCR. The invention also discloses a method for executing fluorescence quantitative RT-PCR for A type, B type and H5 subtype influenza viruses by using the primer and the probe. The detection kit includes RT-PCR reaction buffer, Taq enzyme, inverse transcriptase, DEPC liquid, positive control and negative control. The process involves the steps of establishing a RT-PCR reaction system by using the primer and the probe, extracting DNA or RNA from a specimen to be detected, adding the extracted DNA or RNA into the aforesaid reaction system, setting reaction conditions, carrying out RT-PCR augmentation and fluorescence signal detection. The invention can identify A type, B type and H5 subtype influenza viruses in the detected specimen within 3-4 hours in a fast, exact, specific, safe, simple and convenient way. The invention also has the advantages of quantitative analysis, high sensitivity, being difficult to pollute and wide application scope.