Rapid Detection and Typing of Xanthomonas campestris pv. vesicatoria by Pyrosequencing

Taking Xanthomonas campestris pv.vesicatoria (Doidge) Dye, a pathogen with a wide geographical distribution, as a representative, pyrosequencing is shown for the first time to provide characteristic information of plant pathogenic bacteria strain-specific sequences. Pyrosequencing-based plant pathogen detection and typing technology is demonstrated to be rapid, highly specific and more sensitive than conventional technologies. The specificity of such assays has been validated by conventional DNA sequencing and metabolic fingerprinting. It is a starting point for the application and development of pyrosequencing in plant inspection and quarantine which underlie agricultural communication.