Meta-proteomics Analysis of Microbial Ecosystem in the Anaerobic Digestion of Chicken Manure in Biogas Production Farm

Abstract Using meta-proteomics approach for the analysis of biogas production from anaerobic digestion has many advantages that awaits to be fully uncovered. In this study, we aimed to investigate biogas production from a stable 2-stage chicken manure fermentation system via chemical and biological perspective. The diversity and functional protein changes that occurred from the 1st to 2nd stage were a good indication of exposure on the differential metabolic processes in anaerobic digestion. The highlight of identified functional proteins explained the cause of accumulated ammonia and carbon sources for methane production. Due to the ammonia stress and nutrient limitation, hydrogenotrophic methanogenic pathway was adopted as an indicator of meta-proteomics data involving the key methanogenic substrates (formate and acetate). Unlike traditional meta-genomics analysis, this study identified not only the microorganism's name, but also the enzymes and pathway involved in the generation of methane and carbon dioxide in biogas production of chicken manure.