Influence of the Compatibility of Gualou and Xiebai on Pharmacokinetics of Quercetin in Gualou

Objective: To determine the concentration of quercetin in mouse plasma by HPLC-DAD in different time after oral administration with Gualou Xiebai decoction and Gualou decoction,and to explore the influence of the compatibility of Gualou and Xiebai on pharmacokinetics of quercetin in Gualou. Methods: The plasma sample was simply deproteinized with acetonitrile,extracted three times by aceticether,evaporated to dryness with a gentle N2 stream,dissolved with methanol and determined by HPLC. The pharmacokinetic parameters were separately calculated and compared with data processing software DAS 2. 0 and SPSS 11. 5 software. Results: After oral administration with Gualou Xiebai decoction and Gualou decoction,the pharmacokinetics of quercetin in mouse plasma accorded with the two-compartment model,and t1 /2α,t1 /2β,AUC0-t,AUC0-∞,Cmax,tmax,Ka,K10,K12,K21,CL/F and V1/ F all had significant differences( P 0. 05 or P 0. 01). Conclusion: Xiebai in Gualou Xiebai decoction has a remarkable effect on pharmacokinetic character of quercetin in Gualou,it can promote the absorption and distribution and reduce the elimination of quercetin,in vivo,as well as increase the bioavailability of quercetin.