Influence of ageless liquid with or without piperine on gentamicin-induced nephrotoxicity in wistar rats

To study possible protective role of ageless liquid on gentamicin-induced nephrotoxicity in in SpragueDawley rats. Nephrotoxicity was induced by intraperitoneal injection gentamicin 100 mg/kg/day i.p. to adult Sprague-Dawley rats weighing 1000–250 g. Ageless liquid in three different doses were given for 8 days. On 9th day, blood sample was taken and serum was obtained to assess the levels of urea and creatinine.Thereafter, all the rats were sacrificed by overdose of thiopentone sodium. Following immediate excision of left kidney, it was washed with ice-cold saline to remove as much blood as possible. Renal cortical tissues were separated into two parts for biochemical analysis and light microscopic examination. The gentamicin treated rats have shown a significant increase in MDA, creatinine, urea and a significant decrease in GSH, SOD, catalase and ATP level compared to control rats suggesting the renal damage. The different doses of ageless liquid with piperine and without piperine significantly reversed the renal damage done by gentamicin. The nephroprotective potential was found to be better for ageless liquid with piperine in comparison with ageless liquid without piperine.