Procedure used for denuding pig oocytes influences oocyte damage, and development of in vitro and nuclear transfer embryos

Abstract The effects of different denuding procedures used during the in vitro culture of porcine embryos on oocyte damage and aspects of porcine embryo development were investigated in a series of studies. Oocytes were denuded by vortexing or pipetting after 44 h in vitro maturation (IVM) or pre-denuded after 22 h IVM. The total oocyte death rate was significantly ( P