Aroyl hydrazone with large stokes shift as a fluorescent probe for detection of Cu2+ in pure aqueous medium and in vivo studies

Abstract An aroyl hydrazone based fluorescent probe, hpsh, has been developed for the selective detection of Cu2+ ions in pure aqueous medium by static fluorescence quenching. The fluorescence quenching of hpsh in the presence of Cu2+ takes place as a result of ground state complex formation through intramolecular charge transfer (ICT). Addition of Cu2+ ions changes the color of the solution from colorless to yellow-green which is clearly visible by naked eye. Large Stokes shift of hpsh prevents the self-quenching of the probe in absence of metal ions. The observed stoichiometry between Cu2+ and probe has been found as 1:2 (M: L). MTT assay of hpsh on fruit flies confirms that the probe is non-toxic and biocompatible. The plausible in vivo bioimaging application of the probe to detect Cu2+ in Drosophila gut tissues as well as in adult fruit fly has been investigated with excellent results.