THE DISTRIBUTION AND EXPRESSION OF INTERCELLULAR ADHESI ON MOLECULE-1 ET AL FACTORS OF DC IN THE MURINE S-180 SARCOMA MODEL IN VIVO

To study the migration, distribution and morphological characteristics of DC in vivo, to study the expressions of adhesion molecules and chemokines in the murine S-180 sarcoma model, and their regulative role for DC in the migration. By the method of immunohistochemistry and in situ hybridization stainings, light microscope and electron microscope, We observed the distributive and morphological characteristics of DCs in vivo in the murine S-180 sarcoma model. The expressions of E-cadherin, ICAM-1, MCP-1, Rantes, MIP-1beta mRNA and CCR7 mRNA were compared with control group. The DCs in vivo mainly distributed in the tumor-surrounding tissue and ALN of murine tumor model. The number of DCs was larger in the experiment group than that in control group. The expressions of ICAM-1, MCP-1, Rantes, MIP-1beta mRNA and CCR7 mRNA were higher, but lower of E-cadherin in the murine tumor model. The number and area density of experiment group was greater compared with that of control group. These results suggested that the high expressions of ICAM-1, MCP-1, Rantes, MIP-1beta mRNA and CCR7 mRNA may induce DCs to migrate and gather into lymphatic tissues or tumor-surrounding tissues in the murine tumor model, but the low expressions of E-cadherin may make for the migration of DCs.