Monoclonal antibody, enzyme linked immunosorbent assay and kit for detecting methyltestosterone

The invention discloses a specificity monoclonal antibody capable of distinguishing methyltestosterone. The monoclonal antibody is secreted by a hybridoma cell strain MT9C10 of which the preservation number is CCTCC NO: C201493. The invention further discloses an enzyme linked immunosorbent assay method and kit for detecting the methyltestosterone. Compared with the prior art, the monoclonal antibody disclosed by the invention can be used for distinguishing the methyltestosterone, is high in distinguishing sensitivity and is good in specificity. The ELISA method and the kit, disclosed by the invention, have the advantages of high detection sensitivity, high accuracy and high precision.