Design Approach Regarding Humanization and Functionality of an Anti-CD18 Monoclonal Antibody

In this work the closest human germline sequence was used as the framework on to which to graft murine CDRs. The proposed fully humanized version displays the same staining pattern to different cell subpopulations like CD4, CD8 and memory CD45RO lymphocytes, when compared with the original anti-CD18 MAb, reinforcing the germline approach as a successful strategy to humanize antibodies with maintenance of affinity and selectivity.