Effect of Polysaccharides from Fruit Body of Ganoderma tsugae on Bidirectional Regulation of Proinflammatory Cytokine Production in THP-1 Cells

The polysaccharides,FIII-2,FIII-2-a,FIII-2-b and FIII-2-c were purified from the fruit body of Ganoderma tsugae(G.tsugae) to study the effects of the four polysaccharides on the productions of human proinflammatory cytokines,interleukin-1α(IL-1α) and tumor necrosis factor α(TNFα).The results show that the polysaccharides up-regulated the production of IL-1α and TNFα in THP-1 cells under non-stimulatory condition or under stimulation of both lower concentration lipopolysaccharide(LPS) and phorbol myristate acetate(PMA),and they down-regulated the secretion of IL-1α and TNFα under stronger stimulatory conditions.The four polysaccharides promoted the expression of TNFα mRNA.Production of TNFα protein reflects the quantity of TNFα mRNA.These polysaccharides purified from Ganoderma tsugae bodies exhibit bidirectional regulation effects on cytokine production under different cell stimulatory conditions.