Developing and analysis of a new multiplex panel of eighteen microhaplotypes and compound markers using next generation sequencing and its application to the Shaanxi Han population

Compound marker consists of two different types of genetic markers like deletion/insertion polymorphism (DIP) and single nucleotide polymorphism (SNP) in the genomic region of 200 bp, and microhaplotype consists of a series of closely linked SNPs in a small DNA segment ( 0.1) were observed in six loci which could be further employed for forensic ancestry analyses. Finally, 18 loci were successfully developed into a multiplex panel and detected by the next generation sequencing (NGS) technology. Further analyses of the 18 loci in the studied Shaanxi Han population showed that 15 loci exhibited relatively high expected heterozygosities (>0.5). Cumulative power of discrimination (0.999 999 999 99 4835) of the 18 loci revealed that the multiplex panel could also be utilized for human identifications in the studied Shaanxi Han population. This article is protected by copyright. All rights reserved.