DRUG EFFECTS ON HUMAN LEUCOCYTE MIGRATION AND MIGRATION INHIBITORY ACTIVITY FROM LYMPHOCYTES STIMULATED WITH CONCANAVALIN A

The in vitro effect of a number of drugs on human peripheral blood leucocyte migration and migration inhibitory factors produced by lymphocytes stimulated with concanavalin A was studied. Using the indirect leucocyte migration agarose technique, migration was not influenced by cyclophosphamide, diethylstilbestrol or puromycin. Theophylline (2 μg/ml) marginally inhibited, and methylprednisolone (2.5 μg/ml) markedly stimulated, cell migration under agarose. Production of leucocyte migration inhibitory activity (-LMIA) was inhibited by theophylline (20 and 200 μg/ml) and abolished by methylprednisolone (2.5 μg/ml), cyclophosphamide (2.5 and 25 μg/ml), diethylstilbestrol (0.01 μg/ml) and puromycin (0.1 to 10 μg/ml). The influence of LMIA on migrating cells was inhibited by puromycin (1 and 10 μg/ml) and by high concentrations of diethylstilbestrol (0.1 μg/ml).