Mycobacterium tuberculosis detection kit and use method thereof

The invention provides a mycobacterium tuberculosis detection kit which comprises the following two pairs of primers: inner primers FIP/BIP and outer primers F3/B3, wherein the two pairs of primer takes a gyrB gene of a mycobacterium tuberculosis composite group as a target gene and are designed on the basis of loop-mediated isothermal amplification (LAMP) technology. The mycobacterium tuberculosis detection kit has more comprehensive detection effect and low omission ratio.