Effect of radiation on CBFα1 expression in RANKL-induced osteoclast differentiation of RAW264.7 cells

In order to investigate the changes of the Notch signaling pathway in osteoclast after radiation the mechanism radiation-induced bone loss,the gene expression of CBFα1,the important signal molecular in Notch sig-naling pathway was measured by real-time PCR,respectively.The osteoclast was obtained by RANKL-dependent differentiation in the RAW264.7 cell line.It was found that the 2 Gy 137Cs γ-rays enhanced the CBFα1 expression in RANKL-induced osteoclasts.Taken together,the higher expression of CBFα1 might reinforce the effect of promoting osteoclastogenesis by Notch signaling pathway,increase both of the number and activity of osteoclasts while the bone loss,osteoporosis and fracture occur.