Antibody formation to dog pulp tissue altered by eugenol within the root canal

Pulp tissue from three experimental dogs was tested for its antigenicity before and after incubation with eugenol. Arthus skin test reactions showed an increased response to pulp that had been altered by eugenol and injected as compared with eugenol alone. Hemagglutinating antibody titers (1:360 to 1:600) were obtained to pulp tissue altered by eugenol. Inhibition tests showed the specificity of the antibody to be at a dilution titer of 1/250. Therefore, eugenol altered dog pulp tissue, rendered it antigenically active, and produced a specific humoral response.