Apoptosis of mouse H22 cell cultured in vitro induced with 2-selenium bridged β-cyclodextrin

Objective To study the effects of 2-selenium bridged β-cyclodextrin(2-SeCD) on the apoptosis of H22 cell line and explore its activity of anti-tumor.Methods 2-SeCD with different doses (80,160,320,640 μmol·L-1) were added into the medium in which the cells were cultivated in vitro, negative control group was set up also.The morphological changes of H22 cells were observed under phase contrast microscope;the inhibibory rate of the growth of H22 cells was detected with MTT method,IC50 was calculated,the cell cycle and apoptosis of H22 cells were determined with flow cytometry.Results After exposure of H22 cells to 2-SeCD at the doses of 320 and 640 μmol·L-1for 48 h,the inhibitory rates of H22 cells were(25.90±2.13)% and(49.40±3.66)%,they were higher than that in control group(P0.01).2-SeCD inhibited the growth of H22 cells in dose-dependent manner,and its IC50 was 611.29 μmol·L-1.After exposure of H22 cells to 2-SeCD at the doses of 320 and 640 μmol·L-1 for 48 h,the apoptotic rates were 5.52% and 12.47%,respectively. 2-SeCD inhibited the proliferation of cell cycle at G0/G1 phase.Conclusion 2-SeCD can suppress the growth of H22 cells and induce apoptosis,and it has a potential treatment value in inhibiting hepatoma cell proliferation.