ИСПОЛЬЗОВАНИЕ ЗАЯКОРИВАЮЩИХ МОТИВОВ ДЛЯ ОБЕСПЕЧЕНИЯ ЦЕНТРАЛЬНОЙ ИЛИ ПЕРИФЕРИЧЕСКОЙ ЛОКАЛИЗАЦИИ ОПСИНОВ В ОПТОГЕНЕТИКЕ, "Российский физиологический журнал им. И.М. Сеченова"

One of the ways of optogenetic prosthetics of the degenerative retina is the approach aimed to the reconstruction of the ON/OFF receptive field of ganglionic neurons by targeting the exciting light-activated protein to the central part of the ganglion cell, and the inhibitory one to the periphery. Within the framework of this approach, we investigated the possibility of using various anchoring motifs to provide somatic or dendritic localization of opsins. It has been shown that the Kv2.1 potassium channel motif does not always provide exclusively central localization of the light-activated protein, which may be due to significant overexpression of the construct when strong ubiquitous promoters are used. In addition, we demonstrated the possibility of using one of the proteins that is part of the postsynaptic density, the Homer1 protein, as an anchoring motif for peripheral targeting of rhodopsin