Evaluation of Antioxidant and Antimicrobial Activities of Chitrakadi Vati, a Ayurvedic Formulation

Free radicals are implicated for more than eighty diseases including diabetes mellitus, arthritis, cancer, ageing, etc. in treatment of theses diseases; antioxidant therapy has gained an utmost importance. Current research is now directed towards finding naturally occurring antioxidant of herbal drugs. Antioxidant activity of methanol extract of Chitrakadi Vati was evaluated by using Phosphomolybdenum assay, DPPH radical scavenging assay, superoxide radical scavenging assay and ABTS assay. The total phenolic, total tannins and total flavonoids content were determined. Antibacterial activity was also studied against Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Streptococcus pyogenes by using cup-plate method. Erythromycin was used as standard antibacterial agent. The methanol extract was diluted into different concentration (1, 2, 4, 6, 8, 10 mg/l00 µl) with DMSO. The results of the study revealed that, the Chitrakadi Vati exhibited significant antibacterial activity.