Comparison of Chloroplast DNA psbA-trnH Sequences between Wild and Cultivated Gentiana straminea Maxim.in Qinghai

[Objective] To distinguish Gentiana straminea Maxim. from wild and cultivated plants by comparing chloroplast DNA psbA-trnH sequences, so as to provide a molecular basis for origin identification and quality evaluation.[Method] The cpDNA psbA-trnH sequences of Gentiana straminea Maxim. were amplified by polymerase chain reaction (PCR), and then sequenced by direct PCR sequencing method for homologous analysis. [Result] The lengths of cpDNA psbA-trnH from wild and cultivated plants were 316 and 317 bp respectively, and there were 4 variable sites.[Conclusion] The nucleotide differences of psbA-trnH regions could be used for distinguishing Gentiana straminea Maxim. from wild and cultivated plants.