Integrating Acoustic Perfusion in Mammalian Cell Culture: Temperature Control

The BioSep cell retention system has a virtual (not physical) filter, which avoids clogging (Bierau, et al). The filter in the BioSep system is a field of standing waves, which are formed by exciting a ceramic transducer plate. Part of the energy that is applied to the transducer is transferred into heat. Therefore the BioSep systems should be cooled. The smaller systems (10L and 50L) are air cooled and the larger systems (200L and 1000L) are water cooled. The amount of heat that is produced is depending on the power that is applied to the system. However, the amount of power is not the only influence on the temperature in the system. Other parameters are the harvest flow rate, the temperature of the environment and the length of the recirculation loop. In this study several of these parameters were investigated to determine what settings are optimal for operating the different commercially available BioSep devices.