Herbal tablet of Pueraria tuberosa water extract suppresses the alloxan induced liver damage and hyperglycemia in rats

Background- Liver damage is one of the major metabolic complication of diabetes .As an effective antioxidant  & anti-inflammatory agent ,Pueraria tuberosa could protective diabetic liver. Objective – To study the protective response of herbal formulation (tablets) of Pueraria tuberosa  water extract (PTAB) on alloxan induced rat diabetic model. Material & Method- Alloxan (120 mg/kg bw) was injected intraperitonially. Rats were divided into three groups: group 1 as normal, group 2 as diabetic control and group 3 were given PTAB  upto 14 days. Blood glucose and liver function tests were done using their respective kits. Hematoxylene and eosin staining was done to evaluate the morphological changes in liver tissues. Through immunohistochemistry, we have checked the protein expression of VEGF, MMP9  and ki67. Result – PTAB significantly decreases blood glucose level in a time dependent manner up to 14 days. As compared to diabetic control, PTAB decreases SGOT, SGPT and alkaline phosphates after 14 days of treatment. In diabetic control, the morphology of liver tissues were found damaged due to deformed hepatocytes and dilated lobules. Most of the hepatocytes after PTAB treatment were comparatively found similar to normal rats tissues, along with  dilated blood vessels and normalized liver lobules. In addition to these results,  PTAB suppresses the expressions of VEGF and MMP 9. Conclusion -–PTAB was found to act as an effective hypoglycemic agent. In addition  to this, PTAB  also found  to inhibit inflammation  and apoptosis of liver. Thus, can be taken as a potential drug in liver diseases.  Keywords – Pueraria tuberosa, VEGF, hypoglycaemia, heapatocytes  and liver .