A two-dimensional YACpooling strategy forlibrary screening viaSTSandAIu-PCR methods
1992
We havedeveloped a simple two-dimensional YAC pooling strategy tofacilitate YAClibrary screening via STSandAlu-PCRapproaches. Themethodhasbeen implemented usingthehumantotal genomicYAC library ofOlsonandcoworkers, anditsvalidity tested byisolation ofmany chromosomes 19-and21-specific YACs.TheAlu-PCRapproach isnotable inthatitis hybridization-based, suchthat PCRprimer pairs donot needtoberepeatedly synthesized andtested foreach screening step.
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