High-performance ion-pair chromatography

Isolation of one or more minor components from a sample of biological origin is a very common task in biomedical and biochemical research. The problems met with in this kind of trace analyses are often highly intricate. The trace components must be isolated from a series of other organic compounds of a nature and a concentration which is known to a fairly limited extent. The isolation cannot aim to remove certain impurities; it is instead necessary to base the methodological work on the properties of the analytes and design an isolation procedure that is as specific as possible for these compounds. High-performance liquid chromatography with systems in which it is possible to obtain selective interactions with the objects is often the most efficient approach.