Establishment of a monoclonal antibody directed to the minor novel gangliosides in bovine and cultured neural cell lines

Abstract We established a MAb N-25 reacted with a minor unknown antigen (AgX) in a commercially available GQ1b sample. It also recognized minor antigens in bovine brain (X-1,2,3,4 and 5) and cultured neural cell lines (X-1). AgX is identical to X-5.X-5 is sialidase sensitive and has the common structure as X-1, which is resorcinol positive. These results suggested that novel gangliosides exist in bovine brain and neural cell lines.