Concentración espermática mínima requerida para inseminación intrauterina mediante capacitación por migración ascendente

2014 
6espermatozoides moviles aun en muestras aparentemente inapropiadas caracterizadas por hipospermia u oligozoospermia severa, pero contenian en el total del eyaculado al menos 5 millones de espermatozoides moviles que permitieron un elevado porcentaje de recuperacion espermatica. Conclusiones: La posibilidad de obtener altos porcentajes de recuperacion de espermatozoides moviles en el total del eyaculado permite la inseminacion intrauterina como tecnica de reproduccion asistida en pacientes oligozoospermicos antes de elegir fertilizacion in vitro o inyeccion citoplasmatica del espermatozoide cuando el factor masculino es la causa de infertilidad. Palabras clave: Inseminacion intrauterina. Capacitacion espermatica. Morfologia estricta de Kruger. SUMMARY Objective: To determine what is the minimum necessary amount of motile sperm required for intrauterine insemination and to evaluate the Kruger strict morphology test and sperm motility before and after training sperm by swim up. Methods: Prospective study of 35 semen samples from infertile men, aliquots of 1 mL of fresh semen was washed, centrifuged and over the pellet was placed a layer of capacitation medium to achieve an upward migration. Results: The values of motility and normal sperm forms were observed in the samples significantly increased after the training. It was possible to recover ≥2 x 10 6 motile sperm even in seemingly inappropriate samples with hypospermia or severe oligozoospermia, but these contained in the total ejaculate at least 5 million of motile spermatozoa that allowed a high percentage of retrieval. Conclusions: The possibility of obtaining high recoveries of motile sperm in the total ejaculate allows IUI as assisted reproduction technique in oligozoospermic patients before choosing IVF or cytoplasmic sperm injection when the male factor is cause of infertility.
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