Preparation, characterization and controlled-release property of CS crosslinked MWCNT based on Hericium erinaceus polysaccharides

Abstract In present study, the optimal condition of prepared drug was determined by response surface methodology. In addition, their physicochemical properties, drug release and uptake ability of CS-MWCNT-HEP were studied, and the distribution of the drug in ICR mice and the sites of action were further evaluated. Under the optimal condition, the mean experimental loaded efficiency 68.55±1.47% was corresponded well with the predicted value of 68.28%. The results of in vitro experiments proved that a release of the drug in a pH-dependent behavior. Flow cytometry and inverted microscope showed that the uptake of CS-MWCNT-HEP in Raw264.7 cells increased significantly as the time increased. In vivo experiment proved that the HEP and CS-MWCNT-HEP were mainly accumulated in the kidney, shown the characteristics of kidney metabolism. On the other hand, the extended retention of CS-MWCNT-HEP in the mice could enhance the immune function. CS-MWCNT-HEP has high loaded efficiency and pH-responsive drug released, which could significantly improved the body's immunity and enhance the body's ability to absorbed drugs. These findings proposed a well characterized novel CS-MWCNT-HEP formulation as drug delivery system, and its mechanism and application will be further investigated in our undergoing studies.