Movements and other distinguishing features of small vesicles identified by darkfield microscopy in living macrophages

Abstract Perinuclear vesicles (estimated diameter d -glutamic acid, while the saltations of the ‘larger vesicles’ were markedly slowed; both sets of vesicles stopped after uptake of ammonium chloride. Degranulation of the small vesicles paralleled that of the larger, while simultaneously observed preformed pinosomes (labelled with fluorescent wheat germ agglutinin (WGA) appeared to be unaffected. On the basis also of refractivity, location and speed the ‘small vesicles’ are considered not to be pinosomes, but probably to be lysosomes. The question of whether they are a subgroup of small immature secondary lysosomes or primary lysosomes (0.05–0.08 μm) is discussed. The broad spectrum of movement inhibited by ammonia in macrophages raises the possibility that this weak base inhibits movements of all lysosomes. Further characterization of these ‘small vesicles’ requires their relation to be defined to the small particles in other cell types (especially in axoplasm) which have been detected by video-enhanced microscopy.