Bioeffect of different parameters on four tumor cell lines by ultrasound-mediated microbubble destruction

Objective To explore the bioeffect of different parameters on 4 cell lines by ultrasound-mediated microbubble destruction. Methods The orthogonal experimental design was used to investigate the effect of three factors on the bioeffects of four cell lines under three levels. Three factors included microbubble concentration, sound intensity, irradiation time. Human breast tumor (MCF-7) cells, ovarian tumor (A2780) cells, liver tumor (Bel7402) cells and thyroid tumor (ARO) cells were exposed to ultrasound in the presence of SonoVue. The cell survival rate was determined by MTT methods and the cell luminosity factor was detected by flow cytometry. Results The optimum parameters for Bel7402 and ARO cell were the same(A2B3C2), and they were different from those from MCF-7(A3B1C1) and A2780(A1B3C3) cell. The cell survival rates for 4 cell lines were above 75%, and the cell luminosity factors were different among 4 cell lines. Conclusions The optimum parameters by ultrasound-mediated microbubble destruction for different cell lines are different, and under the optimum parameters the bioeffects of different cell lines are different. Key words: Ultrasonic therapy; Microbubbles; Bioeffect; Cell membrane permeability