An analysis of the features of HBx protein distributed in liver cells and its expression in E. coli.

Objective To investigate the features of HBx protein distributed in liver cells and its expression in E.coli. Methods The expression vectors encoding the full length HBx and its mutants were constructed by the routine molecular cloning method. HBx protein expression was detected using Western blotting. The distribution feature of HBx protein in liver cells was examined using the fluorescence confocal microscopy. A series of purified HBx fusion proteins were obtained by glutathione-sepharose 4B affinity chromatography. Results The expression vectors were successfully constructed for the full length HBx and its mutants. HBx was found distributed uniformly in the nuclei but granularly in the cytoplasm of the liver cells. Under optimal conditions, the mutant GST-HBx (72-120aa) was easily degraded. Conclusion This study may provide a basis for further study on the biological function of HBx at the protein level.