Metabolite Content of Local Bitter White Lupin Seeds (Lupinus albus L.) and Acaricidal and Insecticidal Effects of its Seed Extract

This study investigated the acaricidal and insecticidal effect of local lupin seed extract against Tetranychus urticae Koch, Callosobruchus maculatus F., Plodia interpunctella (Hubner) and metabolite content of seeds. In tests for T. urticae were employed the leaf-disk bioassay. Two μl of the L.albus extract using a micro-applicator were topically applied using a micro-applicator on C. maculatus and P. interpunctella. In the assays of T. urticae , the concentrations of 25, 12.5, 6.25, 3.12, 1.56, 0.78 % (w/w) of the plant extracts were used. Furthermore, the concentrations of 10, 5, 2.50, 1.25, 0.625% (w/w) were applied to P. interpunctella and C. maculatus . Mortality data was collected 24, 48 and 72 hours after application . L. albus extract was found to be quite effective to T. urticae adults with the death rate of 98% mortality in 48 hours and at 12.5% concentrations. The mortality rate (%64) of P. interpunctella larvae were found for the highest value at 72 hours and at 25% concentration. The mortality rate values of C. maculatus adults from L. albus extract at 72 hours were 96, 96 and 100% .at concentrations of %2.5, 5 and 10 %, respectively. In seeds were detected metabolite content, which 686.99 mg GAE / 100 mg total phenol, 22.06 mg QE / 100 mg total flavonoid, DPPH 26.04 mg TE / 100 g antioxidant activity. The toxic effect and bitterness of the plant is due to its high secondary metabolites content. Results indicated that Lupin seed extract had the high toxic effect on T. urticae and C. maculatus.