The use of an IpaC-specific ELISA to identify enteroinvasive Escherichia coli strains of unusual serogroups

Abstract Twenty five Escherichia coli isolates expressing O antigens different from the conventionally recognized enteroinvasive E. coli were tested in the Sereny test, with an invasion plasmid-specific DNA probe, and by an enzyme-linked immunosorbent assay recognizing the secreted IpaC antigen. These results indicate that the IpaC enzyme-linked immunosorbent assay is a sensitive method to recognize enteroinvasive E. coli, irrespective of their serogroups.