A novel tetradecapeptide isolated from bovine adrenal medulla chromaffin vesicles with strong homology to an internal sequence coded by the rat 1B1075 (Preprosecretogranin III) gene

Abstract A novel tetradecapeptide, PheProLysProAlaGlySerGlnAspLysProLeuHisAsn, was isolated from boiling water extracts of bovine adrenal medulla chromaffin vesicles. The primary structure of the peptide was characterized by amino acid analysis, fast atom bombardment mass spectrometry, and gas-phase sequencing. The synthetic and native peptides comigrated on reversed-phase high-performance liquid chromatography, supporting the proposed sequence. This peptide shares 86% homology to residues 67–80 of the recently reported rat 1B1075 gene product secretogranin III and probably represents a processed product derived from the bovine equivalent.