Development of a high-performance liquid chromatography-tandem mass spectrometry method for the determination of flurogestone acetate in ovine plasma
2005
Abstract Flurogestone (FGA) is a synthetic progesterone, with a progestational action higher than that of progesterone itself. It is intended for vaginal use in large animals to induce oestrus synchronization. A quantitative method for the analysis of flurogestone acetate (FGA) in ovine plasma by high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) has been developed. After the incorporation of megestrol acetate (MGA) as internal standard (IS) and followed by a liquid–liquid extraction from plasma, FGA and MGA were chromatographed using a reverse-phase HPLC column and detected by tandem mass spectrometry with a TurboIonSpray ® source. Multiple reaction-monitoring (MRM) mode was used for the quantitative determination of FGA in ovine plasma. The precursor ions [ M + H] + at m / z 407.2 and 385.1 for FGA and MGA, respectively, produced product ions at m / z 267.1/285.1 for FGA and m / z 267.1/224.0 for MGA. The validated concentration range was 0.2–5.0 ng/ml based on 500 μl plasma aliquots. The lower limit of quantitation was 0.2 ng/ml. Fully validated selectivity, accuracy, precision and reproducibility criteria for routine use in pharmacokinetic studies were demonstrated.
Keywords:
- Selected reaction monitoring
- Detection limit
- Flurogestone Acetate
- Analytical chemistry
- Liquid–liquid extraction
- Quantitative analysis (chemistry)
- Tandem mass spectrometry
- High-performance liquid chromatography
- Chemistry
- Chromatography
- Mass spectrometry
- Reproducibility
- Liquid chromatography–mass spectrometry
- Pharmacokinetics
- Correction
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