Combined analysis of P53 Arg72Pro, GSTM1 and GSTT1 gene polymorphisms as predisposing factors for non-small cell lung cancer (NSCLC) development

9581 Background: Gluthatione S-transferase M1 (GSTM1) and T1 (GSTT1) genetic polymorphisms are involved in biotransformation and detoxification of potential carcinogens. Arg72Pro P53 gene polymorphism is suspected to be a predisposing factor for NSCLC development as polymorphic variants of this gene have markedly different apoptotic potential. The aim of this study was to establish the frequencies of the polymorphic Arg72Pro, GSTM1 and GSTT1 genotypes in Polish population and to assess the associated risk of NSCLC development. Methods: Study group included 240 NSCLC patients (52 females and 188 males) who underwent pulmonary resection between the year 1996 and 2000. Control group included 590 healthy subjects. DNA was extracted from frozen blood samples. Genotyping of GSTM1 and GSTT1 genes was performed by PCR-based techniques with co-amplification of human beta globin gene as a positive internal control. Arg72Pro was evaluated by RFLP and DHPLC methods. Results: The proportion of Arg72Pro (pro72 variant)...