Efficacy of Isoniazid and Rifampicin treatment.

CMTB was established in mice and after 2 weeks mice were treated for 6 weeks with Isoniazid (0.1 μg/mL) and Rifampicin (0.1 μg/mL) in the drinking water. After treatment, mice were switched to untreated water for at least 1 week to allow complete clearance of the antibiotics from the mice. (The half-lives of Isoniazid and Rifampicin have been measured to be 4 hours and 2.5 hours). Three mice were sacrificed, and undiluted tissue homogenates of the cervical lymph nodes, lungs, and spleens were plated for CFU measurement. No CFUs were detected after the standard 3-week incubation period. The plot shows CFUs measured after 6 weeks of incubation. (PDF)