Early Identification of Molecular Predictors of Heterotopic Ossification Following Extremity Blast Injury with a Biomarker Assay

Abstract : The purpose of this project is to identify predictive markers of heterotopic ossification in an established animal model that would forecast development of heterotopic ossification (HO) in humans soon after injury. Blast procedures have been completed on all 30 animals (Groups I & II) in the year 1 SOW and 45 animals (Groups III - V) in the year 2 SOW. All animals were biopsied and have been sacrificed according to protocol schedule. Groups I and II animals were also followed with scheduled routine radiographs to monitor progression of HO. Specimen samples are under analysis for gene and protein level expressions with the Nesti partnering molecular biology lab. In year 3, early-appearing gene and protein biomarkers will be identified by analyzing correlations with radiographic HO and will be compared to gene expression signatures in existing human tissue samples known to have gone on to develop HO.