Use of immunoreactive synthetic HTLV-1 peptides in the search for antibody reactivity in multiple sclerosis

The possible association between multiple sclerosis (MS) and antibodies to human T-cell lymphotropic virus type 1 (HTLV-1) was studied. Five synthetic and highly immunoreactive HTLV-1 peptides, four from the envelope (env) region and one from the core (gag) region, were used in an indirect enzyme-linked immunosorbent assay (ELISA). Presence of HTLV-1-specific antibodies in paired serum and cerebrospinal fluid (CSF) samples from 41 MS patients were investigated. No antibody reactivity was demonstrable in samples from 40 of them, whereas one reacted in one serum sample against the gag-peptide. Serum and CSF specimens from 15 with other neurologic diseases (OND), and negative control specimens, i.e. serum and CSF from 9 non-neurologic controls and CSF from 9 healthy controls, did not demonstrate any reactivity in the peptide-ELISAs. Our results do not support involvement of HTLV-1 infection in the etiology of MS.