Recombinant plasmid of expression L-lysine transportprotein and engineering bacteria and application

The invention discloses a recombinant plasmid of expression L-lysine transportprotein and engineering bacteria and application. The gene is from corynebacterium glutamicum ATCC13032, and amplified from a glutamate corynebacterium genome by polymerase chain reaction (PCR); EcoRI and HindIII restriction enzyme cutting sites are respectively added to two ends of the L-lysine transportprotein gene; pKk223-3 connection and transformation after digestion are carried out by using the same restriction enzyme; plasmids are screened and extracted, and electrically converted into the corynebacterium glutamicum ATCC13032; multiplication of the transportprotein in the corynebacterium glutamicum is realized; the molecular weight of the expression product L-lysine transportprotein is 25081.9Da. By adopting the recombinant L-lysine transportprotein or engineering bacteria of expressing the recombinant transportprotein, the L-lysine can be transferred to the outside from inside of the cell, and the recombinant plasmid has the advantages of high transport efficiency and high transfer speed, and the yield of the L-lysine is improved.