The Impact of Statins on FGF–2—Stimulated Human Umbilical Vein Endothelial Cells

AbstractAim: To determine the effects of different types of statins on proliferative and migrative behaviors of basic fibroblastic growth factor (FGF)–2–stimulated endothelial cells. Materials and Methods: Human umbilical vein endothelial cells (HUVECs) were isolated and cultured. Groups were arranged in order to observe the impact of each individual substance alone, or under stimulation with statin on FGF–2–stimulated endothelial cells. Endothelial cells were stimulated with human growth factor (HGF), statins, methyl–β–cyclodextrin (β–MCD), and either farnesyl pyrophosphate (FPP) ammonium salt, or geranylgeranyl–pyrophosphate (GGPP), respectively. Cell proliferation analyses were performed 48 hours after stimulation and gaps between migration borders were used in migration analyses. Results: The statins showed significant antiproliferative and anti–migrative effects and inhibited the proliferative behavior of FGF–2. However, endothelial cell proliferation and migration were significantly increased after ...