红花对兔肺缺血－再灌注损伤时细胞间黏附分子－1表达的影响

Objective To investigate the influence of injection carthamus tinctorius D. (IC) on the expression of intercellular adhesion molecule-1 (ICAM-1) during the ischemia-reperfusion injury of lung (LIRI) in rabbits and its potential mechanism. Method Single lung ischemia-reperfusion animal model was induced in rabbits. A total of 30 Japanese white rabbits were randomly divided into sham-operation group (S group, n=10), ischemiareperfusion group (I/R group, n=10) and ischemia-reperfusion plus IC group (IC group, n=10). The rabbits of IC group received IC 2.0 ml/kg injected intravenously just at 20 min before ligation of artery involved and the same dose of IC instantly at the initiation of reperfusion. Malondialdehyde (MDA), superoxide dismutase (SOD) and xanthine oxidase (XO) in serum were measured. The lung tissue was sampled and assayed wet/dry weight ratio (W/D), contents of myeloperoxidase (MPO) at the end of the experiment, and ultrastructure changes were observed under electron microscope. The expression of ICAM-1 was measured by using immunohistochemistry(IHC). snd one-way ANOVA was used for statistical analysis. Results In I/R group, serum XO and MDA increased and SOD decreased, whereas the same pattern of changes but less magnitude happened in IC group (P＜0.01). The values of W/D and MPO were much higher in I/R group, but lower in IC group. Under electron microscope, the ultrastructure of lung tissue showed pathological changes in the rabbits of I/R group, and these changes were greatly attenuated in the rabbits of IC group. The IHC showed that ICAM-1 in lung tissue of I/R group was (2.94±0.48) which was significantly higher than that of IC group (1.75±0.62) (P＜0.01). Conclusions Injection Carthamus tinctorius D. may meliorate the ischemia-reperfusion injury of lung by way of suppressing the expression of ICAM-1, inhibiting neutrophil aggregation, lowering oxygen free radical level and decreasing lipid peroxidation.