Isolation, production, and characterization of an extracellular lipase from Trichoderma harzianum isolated from soil

Th is is the fi rst report about the characterization of Trichoderma harzianum lipase. A novel strain of Trichoderma harzianum IDM14D was isolated from soil. Th e isolated strain was cultivated for lipase production in shake fl asks at 30 °C for 7 days. For lipase production, it was determined that the best carbon source was glucose and the best nitrogen source was peptone. Maximum biomass was produced at a concentration of 1.25 g/L, in 7 days. Th e optimum pH and temperature for activity of the enzyme were 8.5 and 40 °C, respectively. Th e lipase was stable at a pH range of 8.0-10.0 and at 40 °C for 60 min. Ca 2+ and Mn 2+ enhanced lipase activity but it was determined that other metallic ions did not aff ect the enzyme activity. Th e K m and V max values of the crude enzyme for p-nitrophenyl butyrate hydrolysis were found to be 7.15 mM and 7.067 mM/min, respectively.