A Simple and Rapid Purification of Commercial Trypsin and Chymotrypsin by Reverse-Phase High-Performance Liquid Chromatography**This paper was presented at the International Symposium on HPLC of Proteins and Peptides, November 16–17, 1981, Washington, D. C.

Commercial trypsin and chymotrypsin were further purified with respective recoveries of approximately 80 and 50% of the activity in a reverse-phase high-performance liquid chromatography system using acetonitrile in dilute trifluoroacetic acid at pH 2. The purified enzymes showed single enzymatic activities toward synthetic and protein substrates. The enzymes can be rapidly purified in amounts appropriate for structural analysis of proteins.