Semi-preparative high-performance liquid chromatography in the isolation of derivatives of cardioactive steroidal lactones

Abstract Until now, semi-preparative high-performance liquid chromatography has rarely been applied to cardioactive steroidal lactones and their derivatives. The examples given clearly show the usefulness of the technique for this class of compounds. The technique is superior to open-column and preparative-layer chromatography, especially with respect to speed, separation efficiency and ability to handle sparingly soluble and sensitive compounds.